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    Elucidating the mechanism of cellular uptake and removal of protein

    The possible mechanisms of cellular uptake for the three types of gold nanoparticles were examined, and it was found that different shapes tended to use the various endocytosis pathways in different proportions.

    elucidating the mechanism of cellular uptake and removal of protein-24

    If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Gold nanomaterials have attracted considerable interest as vehicles for intracellular drug delivery.

    We fabricated gold nanostars (GNSs), gold nanorods (GNRs) and gold nanotriangles (GNTs) with similar size and coated them with methylpolyethylene glycol (m PEG) to obtain a neutral surface charge for excluding the interference of other factors (Fig. The m PEG outer layer of m PEG coated gold nanoparticles (P-GNPs) can effectively prevent the adhesion of plasma proteins and subsequent phagocytosis by the immune system; this improves the circulation time of GNPs, the GNPs exhibited distinct color variations.

    The color of GNSs, GNTs, and GNRs is in turn dark green, blue, and wine red.

    At 8h and 24h, shape and time-dependent cellular uptake was observed (Fig. After incubation for 24h, gold concentrations in the cells incubated with P-GNSs, P-GNRs, and P-GNTs were 0.154±0.010pg/cell, 0.814±0.001pg/cell, and 1.333±0.038pg/cell, respectively.

    When the data were converted to percentage uptake from the total added gold the results were 0.38%, 2.04%, and 3.33%.

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